ssr_primer
Primer Details for TRINITY_DN82953_c5_g1_i1 |
|---|
| (T)14(G)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGCCCTCGCTTGACTGAG | GCATGATTTTGATCCCCCGC | Start: 1031 |
| Tm | 59.898 | 59.967 | End: 1149 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | TTTTCCCGGGCCATGTACAA | GCATGATTTTGATCCCCCGC | Start: 979 |
| Tm | 59.887 | 59.967 | End: 1149 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TTTTCCCGGGCCATGTACAA | AAACGGCGTCTAGTGTGGAG | Start: 979 |
| Tm | 59.887 | 60.039 | End: 1097 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
|---|
| Sequence | CTAGCCCTCGCTTGACTGAG | CCGCAACTGGACTATTCCGT | Start: 1031 |
| Tm | 59.898 | 60.108 | End: 1133 |
| Size | 20 | 20 | 103 |
| Primer 5 details |
|---|
| Sequence | TTTTCCCGGGCCATGTACAA | CGGCGTCTAGTGTGGAGTAC | Start: 979 |
| Tm | 59.887 | 59.902 | End: 1094 |
| Size | 20 | 20 | 116 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTAGCCCTCGCTTGACTGAG | GCATGATTTTGATCCCCCGC | Start: 1031 |
| Tm | 59.898 | 59.967 | End: 1149 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | TTTTCCCGGGCCATGTACAA | GCATGATTTTGATCCCCCGC | Start: 979 |
| Tm | 59.887 | 59.967 | End: 1149 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TTTTCCCGGGCCATGTACAA | AAACGGCGTCTAGTGTGGAG | Start: 979 |
| Tm | 59.887 | 60.039 | End: 1097 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
|---|
| Sequence | CTAGCCCTCGCTTGACTGAG | CCGCAACTGGACTATTCCGT | Start: 1031 |
| Tm | 59.898 | 60.108 | End: 1133 |
| Size | 20 | 20 | 103 |
| Primer 5 details |
|---|
| Sequence | TTTTCCCGGGCCATGTACAA | CGGCGTCTAGTGTGGAGTAC | Start: 979 |
| Tm | 59.887 | 59.902 | End: 1094 |
| Size | 20 | 20 | 116 |
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