ssr_primer
Primer Details for TRINITY_DN82940_c5_g1_i1 |
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| (C)12attcgatggggggcaacgagaggggagaggtgaatttttatgaaaccgaagcccctgaaccagctacatgag(C)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCCTCTGCCTGGTTTCAT | TTCACCTCTCCCCTCTCGTT | Start: 323 |
| Tm | 59.887 | 59.885 | End: 450 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
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| Sequence | CCCGTAAAGGCGTGGAGAAA | TGTTAACTGCGCTGACACCT | Start: 236 |
| Tm | 60.321 | 59.894 | End: 383 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
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| Sequence | CCCGTAAAGGCGTGGAGAAA | GCTGTTAACTGCGCTGACAC | Start: 236 |
| Tm | 60.321 | 60.111 | End: 385 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
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| Sequence | CCCGTAAAGGCGTGGAGAAA | GTGCTGTTAACTGCGCTGAC | Start: 236 |
| Tm | 60.321 | 60.111 | End: 387 |
| Size | 20 | 20 | 152 |
| Primer 5 details |
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| Sequence | CCCGTAAAGGCGTGGAGAAA | ATGAAACCAGGCAGAGGCAA | Start: 236 |
| Tm | 60.321 | 59.887 | End: 342 |
| Size | 20 | 20 | 107 |
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