ssr_primer
Primer Details for TRINITY_DN82936_c1_g1_i3 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATGGTGAACAAGCGCTGA | TTGTCTCGATTGGTCTGGCC | Start: 2050 |
| Tm | 59.965 | 60.036 | End: 2183 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | ACATCGGCCATGTTGGTGAT | CAGGACCTCTGTGGCCAATT | Start: 409 |
| Tm | 60.034 | 59.961 | End: 618 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | CACATGGCAGGCAAAGCTTT | CAGGACCTCTGTGGCCAATT | Start: 467 |
| Tm | 59.966 | 59.961 | End: 618 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | TCGGCCATGTTGGTGATTCA | CAGGACCTCTGTGGCCAATT | Start: 412 |
| Tm | 59.963 | 59.961 | End: 618 |
| Size | 20 | 20 | 207 |
| Primer 5 details |
|---|
| Sequence | ACCACCTCGAGCTCAGGTAT | TGCTCCCTTTTGCTCCACAA | Start: 1013 |
| Tm | 60.033 | 60.106 | End: 1131 |
| Size | 20 | 20 | 119 |
(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATGGTGAACAAGCGCTGA | TTGTCTCGATTGGTCTGGCC | Start: 2050 |
| Tm | 59.965 | 60.036 | End: 2183 |
| Size | 20 | 20 | 134 |
| Primer 2 details |
|---|
| Sequence | ACATCGGCCATGTTGGTGAT | CAGGACCTCTGTGGCCAATT | Start: 409 |
| Tm | 60.034 | 59.961 | End: 618 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | CACATGGCAGGCAAAGCTTT | CAGGACCTCTGTGGCCAATT | Start: 467 |
| Tm | 59.966 | 59.961 | End: 618 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | TCGGCCATGTTGGTGATTCA | CAGGACCTCTGTGGCCAATT | Start: 412 |
| Tm | 59.963 | 59.961 | End: 618 |
| Size | 20 | 20 | 207 |
| Primer 5 details |
|---|
| Sequence | ACCACCTCGAGCTCAGGTAT | TGCTCCCTTTTGCTCCACAA | Start: 1013 |
| Tm | 60.033 | 60.106 | End: 1131 |
| Size | 20 | 20 | 119 |
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