ssr_primer
Primer Details for TRINITY_DN82915_c2_g1_i7 |
|---|
| (T)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAAAACAGCGTATGTGCA | GCAACAGTGCCACCAAACTT | Start: 134 |
| Tm | 60.04 | 59.824 | End: 340 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | CTTGCAACAAGGCACCGAAA | GCAACAGTGCCACCAAACTT | Start: 101 |
| Tm | 59.898 | 59.824 | End: 340 |
| Size | 20 | 20 | 240 |
| Primer 3 details |
|---|
| Sequence | AAAACCTACCGGGCAAGAGG | GCAGAAGCCGTCACGAAAAA | Start: 1106 |
| Tm | 59.962 | 59.693 | End: 1344 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | CCGAATCGGTGTTTTTGCGT | ATGTGCGCTCACCTGTTTTG | Start: 483 |
| Tm | 60.041 | 59.688 | End: 725 |
| Size | 20 | 20 | 243 |
| Primer 5 details |
|---|
| Sequence | TGTGACAAGGCGTGAATGGT | TGCCATTTGGACCTTTGGGA | Start: 3507 |
| Tm | 60.179 | 59.81 | End: 3632 |
| Size | 20 | 20 | 126 |
(T)19: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAAAACAGCGTATGTGCA | GCAACAGTGCCACCAAACTT | Start: 134 |
| Tm | 60.04 | 59.824 | End: 340 |
| Size | 20 | 20 | 207 |
| Primer 2 details |
|---|
| Sequence | CTTGCAACAAGGCACCGAAA | GCAACAGTGCCACCAAACTT | Start: 101 |
| Tm | 59.898 | 59.824 | End: 340 |
| Size | 20 | 20 | 240 |
| Primer 3 details |
|---|
| Sequence | AAAACCTACCGGGCAAGAGG | GCAGAAGCCGTCACGAAAAA | Start: 1106 |
| Tm | 59.962 | 59.693 | End: 1344 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | CCGAATCGGTGTTTTTGCGT | ATGTGCGCTCACCTGTTTTG | Start: 483 |
| Tm | 60.041 | 59.688 | End: 725 |
| Size | 20 | 20 | 243 |
| Primer 5 details |
|---|
| Sequence | TGTGACAAGGCGTGAATGGT | TGCCATTTGGACCTTTGGGA | Start: 3507 |
| Tm | 60.179 | 59.81 | End: 3632 |
| Size | 20 | 20 | 126 |
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