ssr_primer
Primer Details for TRINITY_DN82910_c2_g1_i3 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGCTATCGCTACCAGTCC | CACGCACGCACTTGGTAAAA | Start: 79 |
| Tm | 60.04 | 59.972 | End: 278 |
| Size | 20 | 20 | 200 |
| Primer 2 details |
|---|
| Sequence | GGACGCTATCGCTACCAGTC | CACGCACGCACTTGGTAAAA | Start: 78 |
| Tm | 60.04 | 59.972 | End: 278 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | AAGGACCAGAAATCCTGCGG | TTGCCGACGAAAGGGTAGTC | Start: 1277 |
| Tm | 60.035 | 60.038 | End: 1497 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
|---|
| Sequence | GACTACCCTTTCGTCGGCAA | TAGTCGAGTCACACGTCCCT | Start: 1478 |
| Tm | 60.038 | 59.964 | End: 1694 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | AGGGACGTGTGACTCGACTA | CCAGCTGTTTTTGAGCGACC | Start: 1675 |
| Tm | 59.964 | 60.04 | End: 1777 |
| Size | 20 | 20 | 103 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGCTATCGCTACCAGTCC | CACGCACGCACTTGGTAAAA | Start: 79 |
| Tm | 60.04 | 59.972 | End: 278 |
| Size | 20 | 20 | 200 |
| Primer 2 details |
|---|
| Sequence | GGACGCTATCGCTACCAGTC | CACGCACGCACTTGGTAAAA | Start: 78 |
| Tm | 60.04 | 59.972 | End: 278 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | AAGGACCAGAAATCCTGCGG | TTGCCGACGAAAGGGTAGTC | Start: 1277 |
| Tm | 60.035 | 60.038 | End: 1497 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
|---|
| Sequence | GACTACCCTTTCGTCGGCAA | TAGTCGAGTCACACGTCCCT | Start: 1478 |
| Tm | 60.038 | 59.964 | End: 1694 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | AGGGACGTGTGACTCGACTA | CCAGCTGTTTTTGAGCGACC | Start: 1675 |
| Tm | 59.964 | 60.04 | End: 1777 |
| Size | 20 | 20 | 103 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGCTATCGCTACCAGTCC | CACGCACGCACTTGGTAAAA | Start: 79 |
| Tm | 60.04 | 59.972 | End: 278 |
| Size | 20 | 20 | 200 |
| Primer 2 details |
|---|
| Sequence | GGACGCTATCGCTACCAGTC | CACGCACGCACTTGGTAAAA | Start: 78 |
| Tm | 60.04 | 59.972 | End: 278 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | AAGGACCAGAAATCCTGCGG | TTGCCGACGAAAGGGTAGTC | Start: 1277 |
| Tm | 60.035 | 60.038 | End: 1497 |
| Size | 20 | 20 | 221 |
| Primer 4 details |
|---|
| Sequence | GACTACCCTTTCGTCGGCAA | TAGTCGAGTCACACGTCCCT | Start: 1478 |
| Tm | 60.038 | 59.964 | End: 1694 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | AGGGACGTGTGACTCGACTA | CCAGCTGTTTTTGAGCGACC | Start: 1675 |
| Tm | 59.964 | 60.04 | End: 1777 |
| Size | 20 | 20 | 103 |
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