ssr_primer
Primer Details for TRINITY_DN82885_c5_g3_i2 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGTAAAAGCCTGAGAGCCA | CGCAAGCGAACCGGATTATG | Start: 3745 |
| Tm | 60.036 | 60.042 | End: 3943 |
| Size | 20 | 20 | 199 |
| Primer 2 details |
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| Sequence | AAAAGCCTGAGAGCCACCTC | CGCAAGCGAACCGGATTATG | Start: 3749 |
| Tm | 59.963 | 60.042 | End: 3943 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
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| Sequence | TGAGGAGACCACAGGTAGGG | TACACCCCTGTCATCTCCGT | Start: 2696 |
| Tm | 59.959 | 59.959 | End: 2965 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
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| Sequence | GAGGAGACCACAGGTAGGGT | TACACCCCTGTCATCTCCGT | Start: 2697 |
| Tm | 59.959 | 59.959 | End: 2965 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
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| Sequence | ACGGAGATGACAGGGGTGTA | GAAGTTGACGCTAGCGCTTG | Start: 2946 |
| Tm | 59.959 | 59.906 | End: 3048 |
| Size | 20 | 20 | 103 |
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