ssr_primer
Primer Details for TRINITY_DN82846_c0_g3_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTCCAGCACTCTCCGAGA | GCCTGTGAAATTGGCCCAAG | Start: 266 |
| Tm | 59.962 | 60.037 | End: 402 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
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| Sequence | GTGTGCGTGGAGACCTAGAC | AAAGGCTGAGTTCCGAGTCG | Start: 1322 |
| Tm | 60.109 | 60.038 | End: 1549 |
| Size | 20 | 20 | 228 |
| Primer 3 details |
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| Sequence | GGACACCCTCCTCTCCAGAT | GCTCCAGCACCGAGTAATGA | Start: 2177 |
| Tm | 60.032 | 59.824 | End: 2288 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
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| Sequence | GGACACCCTCCTCTCCAGAT | AGCTCCAGCACCGAGTAATG | Start: 2177 |
| Tm | 60.032 | 59.823 | End: 2289 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
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| Sequence | GCGAGAGGTGAACACGATCT | GCCTGTGAAATTGGCCCAAG | Start: 140 |
| Tm | 59.829 | 60.037 | End: 402 |
| Size | 20 | 20 | 263 |
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