ssr_primer
Primer Details for TRINITY_DN82838_c1_g1_i15 |
|---|
| (A)30: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACGGCTGCATTCGTTTTCC | GCATTGGATCGACAGTTGCC | Start: 676 |
| Tm | 60.04 | 59.901 | End: 776 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | AGTATGTGCGGCTCCCAAAA | CCAGGTGAGGTGGAAAACGA | Start: 447 |
| Tm | 59.962 | 59.892 | End: 706 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | AAGTATGTGCGGCTCCCAAA | CCAGGTGAGGTGGAAAACGA | Start: 446 |
| Tm | 59.962 | 59.892 | End: 706 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | TGCTCTCATCGGTGTGAACC | GGCTCACTGAGTTTCCACCA | Start: 1199 |
| Tm | 60.037 | 59.891 | End: 1320 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | CTCTCATCGGTGTGAACCCC | GGCTCACTGAGTTTCCACCA | Start: 1201 |
| Tm | 60.108 | 59.891 | End: 1320 |
| Size | 20 | 20 | 120 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACGGCTGCATTCGTTTTCC | GCATTGGATCGACAGTTGCC | Start: 676 |
| Tm | 60.04 | 59.901 | End: 776 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | AGTATGTGCGGCTCCCAAAA | CCAGGTGAGGTGGAAAACGA | Start: 447 |
| Tm | 59.962 | 59.892 | End: 706 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | AAGTATGTGCGGCTCCCAAA | CCAGGTGAGGTGGAAAACGA | Start: 446 |
| Tm | 59.962 | 59.892 | End: 706 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | TGCTCTCATCGGTGTGAACC | GGCTCACTGAGTTTCCACCA | Start: 1199 |
| Tm | 60.037 | 59.891 | End: 1320 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | CTCTCATCGGTGTGAACCCC | GGCTCACTGAGTTTCCACCA | Start: 1201 |
| Tm | 60.108 | 59.891 | End: 1320 |
| Size | 20 | 20 | 120 |
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