Primer Details for TRINITY_DN82759_c8_g1_i1 | |||
|---|---|---|---|
| (A)18: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCAGAGAAAACCTGGAAATGTCA | CGCCAGATGATTTGAAAGACGT | Start: 22 |
| Tm | 59.178 | 59.583 | End: 176 |
| Size | 24 | 22 | 155 |
| Primer 2 details | |||
| Sequence | GTCAGAGAAAACCTGGAAATGTCA | ACGCCAGATGATTTGAAAGACG | Start: 22 |
| Tm | 59.178 | 59.583 | End: 177 |
| Size | 24 | 22 | 156 |
| Primer 3 details | |||
| Sequence | TCAGAGAAAACCTGGAAATGTCA | CGCCAGATGATTTGAAAGACGT | Start: 23 |
| Tm | 57.952 | 59.583 | End: 176 |
| Size | 23 | 22 | 154 |
| Primer 4 details | |||
| Sequence | TCAGAGAAAACCTGGAAATGTCA | ACGCCAGATGATTTGAAAGACG | Start: 23 |
| Tm | 57.952 | 59.583 | End: 177 |
| Size | 23 | 22 | 155 |
| Primer 5 details | |||
| Sequence | GTCAGAGAAAACCTGGAAATGTCAG | CGCCAGATGATTTGAAAGACGT | Start: 22 |
| Tm | 60.049 | 59.583 | End: 176 |
| Size | 25 | 22 | 155 |
