ssr_primer
Primer Details for TRINITY_DN82742_c2_g1_i13 |
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| (T)23gggggaaaaatgggccggttggaa(G)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCTAACCTCGGGTCAACAA | AAAAGGGCCACTCCACTCAC | Start: 42 |
| Tm | 60.32 | 60.179 | End: 148 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
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| Sequence | GTGAGTGGAGTGGCCCTTTT | CTCCTTGGGGGTTGGTAACG | Start: 129 |
| Tm | 60.179 | 60.322 | End: 405 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
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| Sequence | ATCCTCACCGAGCTACCTCC | CCCATTTTTGGCAAGAGGGC | Start: 295 |
| Tm | 60.468 | 60.036 | End: 424 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
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| Sequence | GGAAAATGCCGCTAACCTCG | AAAAGGGCCACTCCACTCAC | Start: 33 |
| Tm | 59.623 | 60.179 | End: 148 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
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| Sequence | AGATCCTCACCGAGCTACCT | CCCATTTTTGGCAAGAGGGC | Start: 293 |
| Tm | 59.446 | 60.036 | End: 424 |
| Size | 20 | 20 | 132 |
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