ssr_primer
Primer Details for TRINITY_DN82739_c5_g1_i6 |
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| (A)13ctttattgtacctgaattgacctatactgtagcctcacggctcccgtgtatcaaaatgt(AATA)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCTCCAACCTCGTTGAGC | AGGCATTGCAGTCGGATTCA | Start: 739 |
| Tm | 59.966 | 60.035 | End: 894 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
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| Sequence | CAGAGCACAGCCTCCTCAAA | GCTCAACGAGGTTGGAGACA | Start: 645 |
| Tm | 59.964 | 59.966 | End: 758 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
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| Sequence | CTTCCCACAAGAGAACGCCT | AGGTATTGAGTTGTGGGGCG | Start: 1349 |
| Tm | 59.965 | 60.035 | End: 1494 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
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| Sequence | CAGAGCACAGCCTCCTCAAA | AGGCATTGCAGTCGGATTCA | Start: 645 |
| Tm | 59.964 | 60.035 | End: 894 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
|---|
| Sequence | CTTCCCACAAGAGAACGCCT | GGTATTGAGTTGTGGGGCGA | Start: 1349 |
| Tm | 59.965 | 60.036 | End: 1493 |
| Size | 20 | 20 | 145 |
(T)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCTCCAACCTCGTTGAGC | AGGCATTGCAGTCGGATTCA | Start: 739 |
| Tm | 59.966 | 60.035 | End: 894 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | CAGAGCACAGCCTCCTCAAA | GCTCAACGAGGTTGGAGACA | Start: 645 |
| Tm | 59.964 | 59.966 | End: 758 |
| Size | 20 | 20 | 114 |
| Primer 3 details |
|---|
| Sequence | CTTCCCACAAGAGAACGCCT | AGGTATTGAGTTGTGGGGCG | Start: 1349 |
| Tm | 59.965 | 60.035 | End: 1494 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
|---|
| Sequence | CAGAGCACAGCCTCCTCAAA | AGGCATTGCAGTCGGATTCA | Start: 645 |
| Tm | 59.964 | 60.035 | End: 894 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
|---|
| Sequence | CTTCCCACAAGAGAACGCCT | GGTATTGAGTTGTGGGGCGA | Start: 1349 |
| Tm | 59.965 | 60.036 | End: 1493 |
| Size | 20 | 20 | 145 |
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