ssr_primer
Primer Details for TRINITY_DN82683_c0_g1_i1 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGGCGTTAAACGAAAGGG | CATTGTCGACGCGGAGACTA | Start: 243 |
| Tm | 59.836 | 59.902 | End: 479 |
| Size | 20 | 20 | 237 |
| Primer 2 details |
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| Sequence | ACGTTCAAGCGGTCTGGTAG | TTGGCATGGATGTCCGATGT | Start: 1230 |
| Tm | 60.039 | 59.744 | End: 1480 |
| Size | 20 | 20 | 251 |
| Primer 3 details |
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| Sequence | GACGGCGTTAAACGAAAGGG | TTGTCGACGCGGAGACTAAG | Start: 243 |
| Tm | 59.836 | 59.833 | End: 477 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
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| Sequence | ACGGCGTTAAACGAAAGGGA | CATTGTCGACGCGGAGACTA | Start: 244 |
| Tm | 60.25 | 59.902 | End: 479 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
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| Sequence | AAGGAGCCTGACAGGTTTGG | TTGGCATGGATGTCCGATGT | Start: 1265 |
| Tm | 59.889 | 59.744 | End: 1480 |
| Size | 20 | 20 | 216 |
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