ssr_primer
Primer Details for TRINITY_DN82666_c2_g5_i1 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGGAGTTTTGAGGGGTCT | ACAACTGCGTTTTGGACACG | Start: 28 |
| Tm | 60.104 | 59.902 | End: 146 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
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| Sequence | GGGAGTTTTGAGGGGTCTCC | ACAACTGCGTTTTGGACACG | Start: 30 |
| Tm | 59.671 | 59.902 | End: 146 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
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| Sequence | GGGGAGTTTTGAGGGGTCTC | ACAACTGCGTTTTGGACACG | Start: 29 |
| Tm | 59.671 | 59.902 | End: 146 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
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| Sequence | AACGTGAGGTCTTCCCAGTG | ACAACTGCGTTTTGGACACG | Start: 4 |
| Tm | 59.607 | 59.902 | End: 146 |
| Size | 20 | 20 | 143 |
| Primer 5 details |
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| Sequence | AGGGGAGTTTTGAGGGGTCT | TGGGGTGCATCTTAAGGGGA | Start: 28 |
| Tm | 60.104 | 60.551 | End: 210 |
| Size | 20 | 20 | 183 |
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