ssr_primer
Primer Details for TRINITY_DN82631_c1_g3_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCCATGAGCCTAGGGACT | TTTCTGTGAGCTAGCCCTGC | Start: 4727 |
| Tm | 59.955 | 60.036 | End: 4831 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
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| Sequence | AACCCATGAGCCTAGGGACT | CCAAGGCGCCTCAGAAGTAA | Start: 4727 |
| Tm | 59.955 | 60.036 | End: 4856 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
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| Sequence | GGTCAGCCGAGTTTTACCCA | AAGGAGAACCACTGAGGGGT | Start: 3934 |
| Tm | 59.964 | 60.104 | End: 4193 |
| Size | 20 | 20 | 260 |
| Primer 4 details |
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| Sequence | CCCATGAGCCTAGGGACTCT | TTTCTGTGAGCTAGCCCTGC | Start: 4729 |
| Tm | 60.105 | 60.036 | End: 4831 |
| Size | 20 | 20 | 103 |
| Primer 5 details |
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| Sequence | ACCCCTCAGTGGTTCTCCTT | GTAGCCCTGTTTGTCAGCCT | Start: 4174 |
| Tm | 60.104 | 59.963 | End: 4355 |
| Size | 20 | 20 | 182 |
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