ssr_primer
Primer Details for TRINITY_DN82538_c2_g1_i3 |
|---|
| (T)25: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCCCTAGCCATTGACAC | AGCGTGTTTGCCATTGTCAC | Start: 1259 |
| Tm | 60.035 | 59.969 | End: 1525 |
| Size | 20 | 20 | 267 |
| Primer 2 details |
|---|
| Sequence | CCCTGCCAAGATCACCCAAT | CTTGCGAGTTCAGGACAGGT | Start: 2104 |
| Tm | 60.033 | 59.966 | End: 2336 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | CCCTGCCAAGATCACCCAAT | ACTTGCGAGTTCAGGACAGG | Start: 2104 |
| Tm | 60.033 | 59.966 | End: 2337 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | GTGACAATGGCAAACACGCT | ATTTTGGCAGCACGCTCAAG | Start: 1506 |
| Tm | 59.969 | 60.039 | End: 1668 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | GTGACAATGGCAAACACGCT | TTCATTTTGGCAGCACGCTC | Start: 1506 |
| Tm | 59.969 | 60.039 | End: 1671 |
| Size | 20 | 20 | 166 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGCCCTAGCCATTGACAC | AGCGTGTTTGCCATTGTCAC | Start: 1259 |
| Tm | 60.035 | 59.969 | End: 1525 |
| Size | 20 | 20 | 267 |
| Primer 2 details |
|---|
| Sequence | CCCTGCCAAGATCACCCAAT | CTTGCGAGTTCAGGACAGGT | Start: 2104 |
| Tm | 60.033 | 59.966 | End: 2336 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
|---|
| Sequence | CCCTGCCAAGATCACCCAAT | ACTTGCGAGTTCAGGACAGG | Start: 2104 |
| Tm | 60.033 | 59.966 | End: 2337 |
| Size | 20 | 20 | 234 |
| Primer 4 details |
|---|
| Sequence | GTGACAATGGCAAACACGCT | ATTTTGGCAGCACGCTCAAG | Start: 1506 |
| Tm | 59.969 | 60.039 | End: 1668 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
|---|
| Sequence | GTGACAATGGCAAACACGCT | TTCATTTTGGCAGCACGCTC | Start: 1506 |
| Tm | 59.969 | 60.039 | End: 1671 |
| Size | 20 | 20 | 166 |
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