ssr_primer
Primer Details for TRINITY_DN82525_c2_g1_i4 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGTTGGCCGTTTGTCCTG | GATAGTAAGGGCGGCTCGAC | Start: 662 |
| Tm | 59.969 | 60.039 | End: 885 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
|---|
| Sequence | TTTGGTCCCAGCAGATGTCC | ACCAAACCCATCAGCTAGGC | Start: 1485 |
| Tm | 59.962 | 60.034 | End: 1728 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | CGTCTGGTAGCTGTTGCAGA | ACCAAACCCATCAGCTAGGC | Start: 1456 |
| Tm | 60.038 | 60.034 | End: 1728 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | TGAGCGTCTGGTAGCTGTTG | ACCAAACCCATCAGCTAGGC | Start: 1452 |
| Tm | 60.038 | 60.034 | End: 1728 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | GCCTAGCTGATGGGTTTGGT | TGGTGGAAGCATGAGCCATT | Start: 1709 |
| Tm | 60.034 | 59.96 | End: 1840 |
| Size | 20 | 20 | 132 |
(ATTT)4(ATTTTTTTGGAAATTAACTCATTAAGTACTTGCTAGATCTCAATTTAATTTGAAACCTACTTGTTACTGAATTTTTTCATTTTGACACAAGGAAATCTGCTTTCAATAAACAAGAAGTACACATCAAGAAGAATCATATTTGTTATATTTTAAAAGAAATATTCTTCCTATGGTGTATCCTCAATAGAGTTATTGTTCAATGTATCACCGTGATGACTGCAATTCACATCAGAGAATGTTGGCTCCCTGTAAAATTATTTTCTAAGATATTGTTTTGACCGTCTTGAAGTCATTGTCAACACTTTAAATGTATTTTTTTAAGCAGTTTTCTTATTTATGTTGTACAAATTGTCTTTCAGAGTAAACATACCTCTCACCTAATTGAATCATTCTTACATTATTTATTCTCATAGGCCTGTTTGAATCAGTGAGAATGAAAACGCACCAAACAGGAAAAATATAATTTTCAAGAGACACAAAAAACTGCTGAGTGGGTGTA)(T)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGTTGGCCGTTTGTCCTG | GATAGTAAGGGCGGCTCGAC | Start: 662 |
| Tm | 59.969 | 60.039 | End: 885 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
|---|
| Sequence | TTTGGTCCCAGCAGATGTCC | ACCAAACCCATCAGCTAGGC | Start: 1485 |
| Tm | 59.962 | 60.034 | End: 1728 |
| Size | 20 | 20 | 244 |
| Primer 3 details |
|---|
| Sequence | CGTCTGGTAGCTGTTGCAGA | ACCAAACCCATCAGCTAGGC | Start: 1456 |
| Tm | 60.038 | 60.034 | End: 1728 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | TGAGCGTCTGGTAGCTGTTG | ACCAAACCCATCAGCTAGGC | Start: 1452 |
| Tm | 60.038 | 60.034 | End: 1728 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | GCCTAGCTGATGGGTTTGGT | TGGTGGAAGCATGAGCCATT | Start: 1709 |
| Tm | 60.034 | 59.96 | End: 1840 |
| Size | 20 | 20 | 132 |
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