Primer Details for TRINITY_DN82511_c5_g1_i2 |
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| (T)15(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGGGATTGAATAGATTGACATGT | GCTCACAAAGCGCAGACTAA | Start: 71 |
| Tm | 57.853 | 58.852 | End: 173 |
| Size | 25 | 20 | 103 |
| Primer 2 details |
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| Sequence | AGAGGGATTGAATAGATTGACATGT | GCTCACAAAGCGCAGACTAAA | Start: 71 |
| Tm | 57.853 | 59.469 | End: 173 |
| Size | 25 | 21 | 103 |
| Primer 3 details |
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| Sequence | AGGGATTGAATAGATTGACATGTACA | GCTCACAAAGCGCAGACTAA | Start: 73 |
| Tm | 58.195 | 58.852 | End: 173 |
| Size | 26 | 20 | 101 |
| Primer 4 details |
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| Sequence | AGGACATGAATAATAGAGGGATTGA | GCTCACAAAGCGCAGACTAA | Start: 57 |
| Tm | 57.121 | 58.852 | End: 173 |
| Size | 25 | 20 | 117 |
| Primer 5 details |
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| Sequence | GAGGGATTGAATAGATTGACATGTACA | GCTCACAAAGCGCAGACTAA | Start: 72 |
| Tm | 59.066 | 58.852 | End: 173 |
| Size | 27 | 20 | 102 |
