ssr_primer
Primer Details for TRINITY_DN82496_c0_g6_i1 |
|---|
| (T)26: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTGTTCAAAAGCGGCAC | ACCTTGGCAGTTTTCCTGAGT | Start: 139 |
| Tm | 59.934 | 59.785 | End: 274 |
| Size | 19 | 21 | 136 |
| Primer 2 details |
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| Sequence | GGCACGCTAGTTCTTTAGGGT | ACCTTGGCAGTTTTCCTGAGT | Start: 153 |
| Tm | 60.066 | 59.785 | End: 274 |
| Size | 21 | 21 | 122 |
| Primer 3 details |
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| Sequence | GGCACGCTAGTTCTTTAGGG | ACCTTGGCAGTTTTCCTGAGT | Start: 153 |
| Tm | 58.347 | 59.785 | End: 274 |
| Size | 20 | 21 | 122 |
| Primer 4 details |
|---|
| Sequence | TGCTGTTCAAAAGCGGCAC | CCTTGGCAGTTTTCCTGAGT | Start: 139 |
| Tm | 59.934 | 58.013 | End: 273 |
| Size | 19 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | TGCTGTTCAAAAGCGGCAC | ACCTTGGCAGTTTTCCTGAG | Start: 139 |
| Tm | 59.934 | 58.013 | End: 274 |
| Size | 19 | 20 | 136 |
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