ssr_primer
Primer Details for TRINITY_DN82495_c1_g1_i3 |
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| (A)12taaaataaaaaaaaatccgtgtttttcgatttgccagacgttttcctgacacggaaatcgcttgc(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACTGTCGCAGTTTGCCTTC | GTGGCCCCTAAGAGTACACG | Start: 44 |
| Tm | 60.041 | 59.825 | End: 166 |
| Size | 20 | 20 | 123 |
| Primer 2 details |
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| Sequence | AAGCCTCACTGTCGCAGTTT | GTGGCCCCTAAGAGTACACG | Start: 38 |
| Tm | 60.179 | 59.825 | End: 166 |
| Size | 20 | 20 | 129 |
| Primer 3 details |
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| Sequence | CTGTCGCAGTTTGCCTTCAG | GTGGCCCCTAAGAGTACACG | Start: 46 |
| Tm | 59.763 | 59.825 | End: 166 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
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| Sequence | ATAGTGGTATTCAGCGCCGG | GGCATCGCTACTAAGTGGGT | Start: 103 |
| Tm | 59.966 | 59.536 | End: 234 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
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| Sequence | CACTGTCGCAGTTTGCCTTC | GGCATCGCTACTAAGTGGGT | Start: 44 |
| Tm | 60.041 | 59.536 | End: 234 |
| Size | 20 | 20 | 191 |
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