ssr_primer
Primer Details for TRINITY_DN82478_c3_g1_i8 |
|---|
| (A)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGTGAAGCGAATGAAACG | AGGTGCTCAAAGGGCAGATC | Start: 1421 |
| Tm | 60.179 | 60.035 | End: 1594 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | TGTTTTACCTTCCCTCGGCC | ACAGATGATCAGCCACTCGC | Start: 29 |
| Tm | 59.963 | 60.179 | End: 255 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
|---|
| Sequence | TTCCCTCGGCCTTGGTTTTT | ACAGATGATCAGCCACTCGC | Start: 38 |
| Tm | 60.106 | 60.179 | End: 255 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | TTTACCTTCCCTCGGCCTTG | ACAGATGATCAGCCACTCGC | Start: 32 |
| Tm | 59.673 | 60.179 | End: 255 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | GTTTTACCTTCCCTCGGCCT | ACAGATGATCAGCCACTCGC | Start: 30 |
| Tm | 59.673 | 60.179 | End: 255 |
| Size | 20 | 20 | 226 |
(T)10aggcaaaacgtttgtggaaaagttatgaacaactcaaattttgagggtcaatctctcgaatcgtttatttattaa(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGTGAAGCGAATGAAACG | AGGTGCTCAAAGGGCAGATC | Start: 1421 |
| Tm | 60.179 | 60.035 | End: 1594 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | TGTTTTACCTTCCCTCGGCC | ACAGATGATCAGCCACTCGC | Start: 29 |
| Tm | 59.963 | 60.179 | End: 255 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
|---|
| Sequence | TTCCCTCGGCCTTGGTTTTT | ACAGATGATCAGCCACTCGC | Start: 38 |
| Tm | 60.106 | 60.179 | End: 255 |
| Size | 20 | 20 | 218 |
| Primer 4 details |
|---|
| Sequence | TTTACCTTCCCTCGGCCTTG | ACAGATGATCAGCCACTCGC | Start: 32 |
| Tm | 59.673 | 60.179 | End: 255 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | GTTTTACCTTCCCTCGGCCT | ACAGATGATCAGCCACTCGC | Start: 30 |
| Tm | 59.673 | 60.179 | End: 255 |
| Size | 20 | 20 | 226 |
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