ssr_primer
Primer Details for TRINITY_DN82465_c3_g1_i2 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTTGCGGGTAGACAACAT | GCGCTCAAACTGTGTCGTTT | Start: 5387 |
| Tm | 60.035 | 59.972 | End: 5647 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
|---|
| Sequence | TGCGGGTAGACAACATTGCT | GCGCTCAAACTGTGTCGTTT | Start: 5391 |
| Tm | 59.964 | 59.972 | End: 5647 |
| Size | 20 | 20 | 257 |
| Primer 3 details |
|---|
| Sequence | TTCCATCTGCTCCGGGAAAC | TCCTACGTATTCCCCTGCCA | Start: 3308 |
| Tm | 60.035 | 60.032 | End: 3469 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TCGGAAGACCACGACAAAGG | TACGTTTGGCAGCTGGATGT | Start: 2060 |
| Tm | 59.967 | 59.964 | End: 2233 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | AGTTGCTTGAGGGACGTCTG | TGTCAGCACCGCTAAGAAGG | Start: 4148 |
| Tm | 59.966 | 60.037 | End: 4319 |
| Size | 20 | 20 | 172 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCTTGCGGGTAGACAACAT | GCGCTCAAACTGTGTCGTTT | Start: 5387 |
| Tm | 60.035 | 59.972 | End: 5647 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
|---|
| Sequence | TGCGGGTAGACAACATTGCT | GCGCTCAAACTGTGTCGTTT | Start: 5391 |
| Tm | 59.964 | 59.972 | End: 5647 |
| Size | 20 | 20 | 257 |
| Primer 3 details |
|---|
| Sequence | TTCCATCTGCTCCGGGAAAC | TCCTACGTATTCCCCTGCCA | Start: 3308 |
| Tm | 60.035 | 60.032 | End: 3469 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TCGGAAGACCACGACAAAGG | TACGTTTGGCAGCTGGATGT | Start: 2060 |
| Tm | 59.967 | 59.964 | End: 2233 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | AGTTGCTTGAGGGACGTCTG | TGTCAGCACCGCTAAGAAGG | Start: 4148 |
| Tm | 59.966 | 60.037 | End: 4319 |
| Size | 20 | 20 | 172 |
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