ssr_primer
Primer Details for TRINITY_DN82452_c8_g2_i1 |
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| (A)30: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTTCGGTATCATCGGCACC | AGAGGTTGGAAGGAATGGCG | Start: 406 |
| Tm | 60.179 | 60.035 | End: 519 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
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| Sequence | AGCGCTGAGGACGAAGAATC | AGAGGTTGGAAGGAATGGCG | Start: 375 |
| Tm | 60.179 | 60.035 | End: 519 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
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| Sequence | GCCACACTTGCACTGCATTT | AGAGGTTGGAAGGAATGGCG | Start: 306 |
| Tm | 60.25 | 60.035 | End: 519 |
| Size | 20 | 20 | 214 |
| Primer 4 details |
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| Sequence | CTCAAGGTGAAGTTGGCGGA | AGAGGTTGGAAGGAATGGCG | Start: 339 |
| Tm | 60.25 | 60.035 | End: 519 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
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| Sequence | TGTTCGGTATCATCGGCACC | TTGGAAGGAATGGCGGGAAA | Start: 406 |
| Tm | 60.179 | 59.887 | End: 514 |
| Size | 20 | 20 | 109 |
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