ssr_primer
Primer Details for TRINITY_DN82438_c1_g1_i6 |
|---|
| (AG)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGAAGGATACGAGGCTGT | CATGCACGAGATTTCCGCTG | Start: 565 |
| Tm | 60.032 | 59.973 | End: 839 |
| Size | 20 | 20 | 275 |
| Primer 2 details |
|---|
| Sequence | TCCGCTCGGATCACAACAAA | GTCCGCCCTCCTATTTCTCG | Start: 1232 |
| Tm | 59.966 | 59.968 | End: 1449 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | CGAGAAATAGGAGGGCGGAC | TGACATCATGTTCTGCCGCT | Start: 1430 |
| Tm | 59.968 | 60.036 | End: 1618 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | CCGATACCTCTCCTTTCCGC | TTCCGCATGAGGTTGAGGTC | Start: 2552 |
| Tm | 59.968 | 60.036 | End: 2828 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | TCCGCTCGGATCACAACAAA | TTTCGATCTGACCTTGGCCC | Start: 1232 |
| Tm | 59.966 | 60.035 | End: 1498 |
| Size | 20 | 20 | 267 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGAAGGATACGAGGCTGT | CATGCACGAGATTTCCGCTG | Start: 565 |
| Tm | 60.032 | 59.973 | End: 839 |
| Size | 20 | 20 | 275 |
| Primer 2 details |
|---|
| Sequence | TCCGCTCGGATCACAACAAA | GTCCGCCCTCCTATTTCTCG | Start: 1232 |
| Tm | 59.966 | 59.968 | End: 1449 |
| Size | 20 | 20 | 218 |
| Primer 3 details |
|---|
| Sequence | CGAGAAATAGGAGGGCGGAC | TGACATCATGTTCTGCCGCT | Start: 1430 |
| Tm | 59.968 | 60.036 | End: 1618 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
|---|
| Sequence | CCGATACCTCTCCTTTCCGC | TTCCGCATGAGGTTGAGGTC | Start: 2552 |
| Tm | 59.968 | 60.036 | End: 2828 |
| Size | 20 | 20 | 277 |
| Primer 5 details |
|---|
| Sequence | TCCGCTCGGATCACAACAAA | TTTCGATCTGACCTTGGCCC | Start: 1232 |
| Tm | 59.966 | 60.035 | End: 1498 |
| Size | 20 | 20 | 267 |
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