ssr_primer
Primer Details for TRINITY_DN82354_c2_g1_i1 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCTCTGCTGCAGGAGACG | AAGTGACACACGCTGAAGGT | Start: 571 |
| Tm | 60.038 | 59.822 | End: 726 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
|---|
| Sequence | TTTCTCTGCTGCAGGAGACG | ACGCTGAAGGTCGTTCCAAA | Start: 571 |
| Tm | 60.038 | 60.179 | End: 717 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | TTTCTCTGCTGCAGGAGACG | AGTGACACACGCTGAAGGTC | Start: 571 |
| Tm | 60.038 | 60.249 | End: 725 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | AGGAGACGGGAGACACAGAA | AAGTGACACACGCTGAAGGT | Start: 583 |
| Tm | 59.887 | 59.822 | End: 726 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | AGGAGACGGGAGACACAGAA | ACGCTGAAGGTCGTTCCAAA | Start: 583 |
| Tm | 59.887 | 60.179 | End: 717 |
| Size | 20 | 20 | 135 |
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