ssr_primer
Primer Details for TRINITY_DN82339_c0_g1_i2 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAACCTCTCAAGTTCCGCC | GCCTCAAGTTGCGCAAGAAA | Start: 1300 |
| Tm | 59.965 | 59.969 | End: 1573 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
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| Sequence | CCCTCCATCGTCCTCCCTAA | CCCAATGGCCGACCTAAGTT | Start: 779 |
| Tm | 60.106 | 60.034 | End: 981 |
| Size | 20 | 20 | 203 |
| Primer 3 details |
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| Sequence | AACTTAGGTCGGCCATTGGG | GGTCTCCCGAACCCAGTTTT | Start: 962 |
| Tm | 60.034 | 59.89 | End: 1238 |
| Size | 20 | 20 | 277 |
| Primer 4 details |
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| Sequence | CGTGCCATCGGTAAAGGAGA | GCCTCAAGTTGCGCAAGAAA | Start: 1353 |
| Tm | 59.825 | 59.969 | End: 1573 |
| Size | 20 | 20 | 221 |
| Primer 5 details |
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| Sequence | AACTTAGGTCGGCCATTGGG | TTCAGAATGCTCTCGACGGG | Start: 962 |
| Tm | 60.034 | 59.827 | End: 1179 |
| Size | 20 | 20 | 218 |
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