ssr_primer
Primer Details for TRINITY_DN82336_c5_g1_i1 |
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| (A)28: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGGCTGGACAAACTACAGT | CCGCCGATACTCTTCAGACC | Start: 1937 |
| Tm | 59.965 | 59.969 | End: 2152 |
| Size | 20 | 20 | 216 |
| Primer 2 details |
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| Sequence | CGGGCTGGACAAACTACAGT | CCCGCCGATACTCTTCAGAC | Start: 1937 |
| Tm | 59.965 | 59.969 | End: 2153 |
| Size | 20 | 20 | 217 |
| Primer 3 details |
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| Sequence | CGGGCTGGACAAACTACAGT | ATTGTTGTTTTCACCCGCCG | Start: 1937 |
| Tm | 59.965 | 59.969 | End: 2166 |
| Size | 20 | 20 | 230 |
| Primer 4 details |
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| Sequence | GCTAGGTAGAGTCGCAGCAG | ACCAGGCGGAGTTTACGAAG | Start: 1175 |
| Tm | 59.97 | 60.038 | End: 1363 |
| Size | 20 | 20 | 189 |
| Primer 5 details |
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| Sequence | AAGGCTCCGGAATCCTTTGG | TTAACCTGGGTCTTGACGCC | Start: 1577 |
| Tm | 60.034 | 59.964 | End: 1827 |
| Size | 20 | 20 | 251 |
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