ssr_primer
Primer Details for TRINITY_DN82309_c2_g2_i1 |
|---|
| (T)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTTCAAACCTCCGCGTCT | CGACTCCGACGGAAAAAGGA | Start: 241 |
| Tm | 59.894 | 60.039 | End: 418 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | TCCTTTTTCCGTCGGAGTCG | AGATTTTCCGGCGACTGGAG | Start: 399 |
| Tm | 60.039 | 60.108 | End: 507 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | ACTTGTGCCAAACCGCAATC | CTTCTCTTCCGCAGCTACGT | Start: 1568 |
| Tm | 59.968 | 59.829 | End: 1761 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
|---|
| Sequence | GACTTGTGCCAAACCGCAAT | CTTCTCTTCCGCAGCTACGT | Start: 1567 |
| Tm | 59.968 | 59.829 | End: 1761 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
|---|
| Sequence | ACTTTCAAACCTCCGCGTCT | AGATTTTCCGGCGACTGGAG | Start: 241 |
| Tm | 59.894 | 60.108 | End: 507 |
| Size | 20 | 20 | 267 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTTCAAACCTCCGCGTCT | CGACTCCGACGGAAAAAGGA | Start: 241 |
| Tm | 59.894 | 60.039 | End: 418 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | TCCTTTTTCCGTCGGAGTCG | AGATTTTCCGGCGACTGGAG | Start: 399 |
| Tm | 60.039 | 60.108 | End: 507 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | ACTTGTGCCAAACCGCAATC | CTTCTCTTCCGCAGCTACGT | Start: 1568 |
| Tm | 59.968 | 59.829 | End: 1761 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
|---|
| Sequence | GACTTGTGCCAAACCGCAAT | CTTCTCTTCCGCAGCTACGT | Start: 1567 |
| Tm | 59.968 | 59.829 | End: 1761 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
|---|
| Sequence | ACTTTCAAACCTCCGCGTCT | AGATTTTCCGGCGACTGGAG | Start: 241 |
| Tm | 59.894 | 60.108 | End: 507 |
| Size | 20 | 20 | 267 |
(A)46: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTTCAAACCTCCGCGTCT | CGACTCCGACGGAAAAAGGA | Start: 241 |
| Tm | 59.894 | 60.039 | End: 418 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | TCCTTTTTCCGTCGGAGTCG | AGATTTTCCGGCGACTGGAG | Start: 399 |
| Tm | 60.039 | 60.108 | End: 507 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | ACTTGTGCCAAACCGCAATC | CTTCTCTTCCGCAGCTACGT | Start: 1568 |
| Tm | 59.968 | 59.829 | End: 1761 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
|---|
| Sequence | GACTTGTGCCAAACCGCAAT | CTTCTCTTCCGCAGCTACGT | Start: 1567 |
| Tm | 59.968 | 59.829 | End: 1761 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
|---|
| Sequence | ACTTTCAAACCTCCGCGTCT | AGATTTTCCGGCGACTGGAG | Start: 241 |
| Tm | 59.894 | 60.108 | End: 507 |
| Size | 20 | 20 | 267 |
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