ssr_primer
Primer Details for TRINITY_DN82291_c1_g1_i2 |
|---|
| (A)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTTCATCGAATTTCGGCC | TGGCTCATCCGGAAACTCAC | Start: 1038 |
| Tm | 59.971 | 60.036 | End: 1226 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | GCTGTGAGTTGTGAACTGCG | GGCCGAAATTCGATGAAGCC | Start: 845 |
| Tm | 60.042 | 59.971 | End: 1057 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
|---|
| Sequence | TCTCTTCGGCAAATCGGCTT | TGGCTCATCCGGAAACTCAC | Start: 1023 |
| Tm | 60.036 | 60.036 | End: 1226 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | AGCACGCACTTACTGCTCTT | CAAACTACAACCCCCTCCCC | Start: 1266 |
| Tm | 59.966 | 59.961 | End: 1404 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | GTGAGTTTCCGGATGAGCCA | CAAACTACAACCCCCTCCCC | Start: 1207 |
| Tm | 60.036 | 59.961 | End: 1404 |
| Size | 20 | 20 | 198 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTTCATCGAATTTCGGCC | TGGCTCATCCGGAAACTCAC | Start: 1038 |
| Tm | 59.971 | 60.036 | End: 1226 |
| Size | 20 | 20 | 189 |
| Primer 2 details |
|---|
| Sequence | GCTGTGAGTTGTGAACTGCG | GGCCGAAATTCGATGAAGCC | Start: 845 |
| Tm | 60.042 | 59.971 | End: 1057 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
|---|
| Sequence | TCTCTTCGGCAAATCGGCTT | TGGCTCATCCGGAAACTCAC | Start: 1023 |
| Tm | 60.036 | 60.036 | End: 1226 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | AGCACGCACTTACTGCTCTT | CAAACTACAACCCCCTCCCC | Start: 1266 |
| Tm | 59.966 | 59.961 | End: 1404 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | GTGAGTTTCCGGATGAGCCA | CAAACTACAACCCCCTCCCC | Start: 1207 |
| Tm | 60.036 | 59.961 | End: 1404 |
| Size | 20 | 20 | 198 |
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