ssr_primer
Primer Details for TRINITY_DN82261_c3_g2_i3 |
|---|
| (T)25: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACCGCATCTGAGGATTCGC | TTCCGTGGAATGGGGAAGTG | Start: 2235 |
| Tm | 59.968 | 59.962 | End: 2371 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
|---|
| Sequence | CACTTCCCCATTCCACGGAA | TGCTTAGCTGGGTGCATTGA | Start: 2352 |
| Tm | 59.962 | 59.962 | End: 2587 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
|---|
| Sequence | GGCCACGGAACACAAATTCC | GCCCTGCATGGTGACTTTTG | Start: 3548 |
| Tm | 60.039 | 60.038 | End: 3752 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | CACTTCCCCATTCCACGGAA | CAGCCGCTGAATTTGTCTCG | Start: 2352 |
| Tm | 59.962 | 59.904 | End: 2518 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACGCAACAGCTGGAAGAGAA | CACTTTGGTCTCCGACTGCT | Start: 2872 |
| Tm | 59.893 | 59.966 | End: 3082 |
| Size | 20 | 20 | 211 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACCGCATCTGAGGATTCGC | TTCCGTGGAATGGGGAAGTG | Start: 2235 |
| Tm | 59.968 | 59.962 | End: 2371 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
|---|
| Sequence | CACTTCCCCATTCCACGGAA | TGCTTAGCTGGGTGCATTGA | Start: 2352 |
| Tm | 59.962 | 59.962 | End: 2587 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
|---|
| Sequence | GGCCACGGAACACAAATTCC | GCCCTGCATGGTGACTTTTG | Start: 3548 |
| Tm | 60.039 | 60.038 | End: 3752 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | CACTTCCCCATTCCACGGAA | CAGCCGCTGAATTTGTCTCG | Start: 2352 |
| Tm | 59.962 | 59.904 | End: 2518 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
|---|
| Sequence | ACGCAACAGCTGGAAGAGAA | CACTTTGGTCTCCGACTGCT | Start: 2872 |
| Tm | 59.893 | 59.966 | End: 3082 |
| Size | 20 | 20 | 211 |
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