ssr_primer
Primer Details for TRINITY_DN82253_c5_g1_i7 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCACGAGGTTGTTGGATGA | ACCGCAACTCAAGTTAGGGT | Start: 81 |
| Tm | 59.604 | 59.237 | End: 211 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
|---|
| Sequence | AGCACGAGGTTGTTGGATGA | AACCGCAACTCAAGTTAGGGT | Start: 81 |
| Tm | 59.604 | 59.858 | End: 212 |
| Size | 20 | 21 | 132 |
| Primer 3 details |
|---|
| Sequence | AGCACGAGGTTGTTGGATGAT | ACCGCAACTCAAGTTAGGGT | Start: 81 |
| Tm | 59.996 | 59.237 | End: 211 |
| Size | 21 | 20 | 131 |
| Primer 4 details |
|---|
| Sequence | AGCACGAGGTTGTTGGATGA | TGGTAGAACCGCAACTCAAGT | Start: 81 |
| Tm | 59.604 | 59.585 | End: 218 |
| Size | 20 | 21 | 138 |
| Primer 5 details |
|---|
| Sequence | CCGAACAACGAATTCTGCCTG | ACCGCAACTCAAGTTAGGGT | Start: 11 |
| Tm | 60.136 | 59.237 | End: 211 |
| Size | 21 | 20 | 201 |
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