ssr_primer
Primer Details for TRINITY_DN82224_c4_g5_i1 |
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| (T)27: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCACGACGCTTTTGGATG | CTTACCTGAAGACTCGCCGG | Start: 55 |
| Tm | 60.179 | 60.179 | End: 246 |
| Size | 20 | 20 | 192 |
| Primer 2 details |
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| Sequence | TTTTCTTTCCCCGCCTGGAA | CTTACCTGAAGACTCGCCGG | Start: 143 |
| Tm | 59.814 | 60.179 | End: 246 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
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| Sequence | TTTTTCTTTCCCCGCCTGGA | CTTACCTGAAGACTCGCCGG | Start: 142 |
| Tm | 59.814 | 60.179 | End: 246 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
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| Sequence | GCTCACGACGCTTTTGGATG | TCCAGGCGGGGAAAGAAAAA | Start: 55 |
| Tm | 60.179 | 59.814 | End: 161 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
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| Sequence | GCTCACGACGCTTTTGGATG | TTCCAGGCGGGGAAAGAAAA | Start: 55 |
| Tm | 60.179 | 59.814 | End: 162 |
| Size | 20 | 20 | 108 |
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