ssr_primer
Primer Details for TRINITY_DN82212_c1_g1_i2 |
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| (T)25aa(T)10aaaattaacttttcatttttcc(T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGATCCCTTACATCGCACCG | AATTCCGGACGCGTTTTCAA | Start: 24 |
| Tm | 59.611 | 59.054 | End: 171 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
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| Sequence | CCCTTACATCGCACCGGTAT | AATTCCGGACGCGTTTTCAA | Start: 28 |
| Tm | 59.608 | 59.054 | End: 171 |
| Size | 20 | 20 | 144 |
| Primer 3 details |
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| Sequence | GATCCCTTACATCGCACCGG | AATTCCGGACGCGTTTTCAA | Start: 25 |
| Tm | 60.6 | 59.054 | End: 171 |
| Size | 20 | 20 | 147 |
| Primer 4 details |
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| Sequence | AGATCCCTTACATCGCACCG | ATTCCGGACGCGTTTTCAAT | Start: 24 |
| Tm | 59.611 | 58.838 | End: 170 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
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| Sequence | CCCTTACATCGCACCGGTAT | ATTCCGGACGCGTTTTCAAT | Start: 28 |
| Tm | 59.608 | 58.838 | End: 170 |
| Size | 20 | 20 | 143 |
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