ssr_primer
Primer Details for TRINITY_DN82185_c1_g2_i5 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCCGTCAGAAGTCAACTC | AAGAAAACGTGTTCAGCGGC | Start: 113 |
| Tm | 59.966 | 59.971 | End: 350 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | CTGGCCGTCAGAAGTCAACT | AAGAAAACGTGTTCAGCGGC | Start: 112 |
| Tm | 59.966 | 59.971 | End: 350 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | ATACATGGCAGCTTGCTCGT | GCCCTTGGAGTCGCTTTTTG | Start: 944 |
| Tm | 60.108 | 60.039 | End: 1059 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | GCCGCTGAACACGTTTTCTT | AGAACTTGGCACGTGAACGA | Start: 331 |
| Tm | 59.971 | 60.179 | End: 479 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | ATACATGGCAGCTTGCTCGT | CAAAATCGCGAACGCTCACA | Start: 944 |
| Tm | 60.108 | 60.111 | End: 1107 |
| Size | 20 | 20 | 164 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCCGTCAGAAGTCAACTC | AAGAAAACGTGTTCAGCGGC | Start: 113 |
| Tm | 59.966 | 59.971 | End: 350 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
|---|
| Sequence | CTGGCCGTCAGAAGTCAACT | AAGAAAACGTGTTCAGCGGC | Start: 112 |
| Tm | 59.966 | 59.971 | End: 350 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | ATACATGGCAGCTTGCTCGT | GCCCTTGGAGTCGCTTTTTG | Start: 944 |
| Tm | 60.108 | 60.039 | End: 1059 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | GCCGCTGAACACGTTTTCTT | AGAACTTGGCACGTGAACGA | Start: 331 |
| Tm | 59.971 | 60.179 | End: 479 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | ATACATGGCAGCTTGCTCGT | CAAAATCGCGAACGCTCACA | Start: 944 |
| Tm | 60.108 | 60.111 | End: 1107 |
| Size | 20 | 20 | 164 |
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