ssr_primer
Primer Details for TRINITY_DN82177_c4_g1_i2 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATCGGGAAGCTTTGAGCA | CCTCAATTTGGAGCCGGTCT | Start: 1108 |
| Tm | 59.963 | 60.035 | End: 1325 |
| Size | 20 | 20 | 218 |
| Primer 2 details |
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| Sequence | ACATCGGGAAGCTTTGAGCA | AGACCTCAATTTGGAGCCGG | Start: 1108 |
| Tm | 59.963 | 60.035 | End: 1328 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
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| Sequence | CAACACAATAGCCACCGCAG | TTGGGAGTCTTCAGTGACGC | Start: 301 |
| Tm | 59.831 | 59.966 | End: 542 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
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| Sequence | AGACTCATGCCAGCTGTGAC | CTGCGGTGGCTATTGTGTTG | Start: 143 |
| Tm | 60.037 | 59.831 | End: 320 |
| Size | 20 | 20 | 178 |
| Primer 5 details |
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| Sequence | ATAGCCACCGCAGTTCTGAG | TTGGGAGTCTTCAGTGACGC | Start: 308 |
| Tm | 59.823 | 59.966 | End: 542 |
| Size | 20 | 20 | 235 |
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