ssr_primer
Primer Details for TRINITY_DN82174_c4_g1_i1 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCAATTCAAGGCGCCTGC | TGTTGCCAGCTGGTACGTAG | Start: 164 |
| Tm | 60.038 | 60.038 | End: 398 |
| Size | 20 | 20 | 235 |
| Primer 2 details |
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| Sequence | TCAGTGCCCTCTCAACCAAC | TGTTGCCAGCTGGTACGTAG | Start: 276 |
| Tm | 59.891 | 60.038 | End: 398 |
| Size | 20 | 20 | 123 |
| Primer 3 details |
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| Sequence | TTTCAATTCAAGGCGCCTGC | GTTGGTTGAGAGGGCACTGA | Start: 164 |
| Tm | 60.038 | 59.891 | End: 295 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
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| Sequence | AGCCGCAGACAATAGAGCAG | TTCCACGGTCCCGAATTCAG | Start: 628 |
| Tm | 60.179 | 60.037 | End: 872 |
| Size | 20 | 20 | 245 |
| Primer 5 details |
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| Sequence | TTTCAATTCAAGGCGCCTGC | TGGTTTTTGTTGCCAGCTGG | Start: 164 |
| Tm | 60.038 | 59.822 | End: 405 |
| Size | 20 | 20 | 242 |
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