ssr_primer
Primer Details for TRINITY_DN82118_c3_g1_i5 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTCTCCTGCAACCCGAAA | GAAGCCGATGGTGGAGACAA | Start: 1486 |
| Tm | 59.965 | 60.036 | End: 1640 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | GACTCTCCTGCAACCCGAAA | TTGAAGCCGATGGTGGAGAC | Start: 1486 |
| Tm | 59.965 | 60.036 | End: 1642 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | GTCTCCACCATCGGCTTCAA | TGCTCGAGCCTGTGTTCAAT | Start: 1623 |
| Tm | 60.036 | 59.965 | End: 1870 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | TTGTCTCCACCATCGGCTTC | TGCTCGAGCCTGTGTTCAAT | Start: 1621 |
| Tm | 60.036 | 59.965 | End: 1870 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
|---|
| Sequence | GAATGCGGTCGCCAAACTTT | TAGATGGGGCGGGCAATTTT | Start: 2467 |
| Tm | 60.04 | 60.033 | End: 2715 |
| Size | 20 | 20 | 249 |
(A)12ttaaaaaaggtaaatttgttagaattattaaagaattattcgagaagttaataaagaaaatattttgtaatc(A)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTCTCCTGCAACCCGAAA | GAAGCCGATGGTGGAGACAA | Start: 1486 |
| Tm | 59.965 | 60.036 | End: 1640 |
| Size | 20 | 20 | 155 |
| Primer 2 details |
|---|
| Sequence | GACTCTCCTGCAACCCGAAA | TTGAAGCCGATGGTGGAGAC | Start: 1486 |
| Tm | 59.965 | 60.036 | End: 1642 |
| Size | 20 | 20 | 157 |
| Primer 3 details |
|---|
| Sequence | GTCTCCACCATCGGCTTCAA | TGCTCGAGCCTGTGTTCAAT | Start: 1623 |
| Tm | 60.036 | 59.965 | End: 1870 |
| Size | 20 | 20 | 248 |
| Primer 4 details |
|---|
| Sequence | TTGTCTCCACCATCGGCTTC | TGCTCGAGCCTGTGTTCAAT | Start: 1621 |
| Tm | 60.036 | 59.965 | End: 1870 |
| Size | 20 | 20 | 250 |
| Primer 5 details |
|---|
| Sequence | GAATGCGGTCGCCAAACTTT | TAGATGGGGCGGGCAATTTT | Start: 2467 |
| Tm | 60.04 | 60.033 | End: 2715 |
| Size | 20 | 20 | 249 |
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