ssr_primer
Primer Details for TRINITY_DN82102_c2_g1_i3 |
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| (T)35: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGGAAAGGGGCCTAAAACC | ATGCTACGGAAGAACGCTGT | Start: 171 |
| Tm | 59.961 | 59.753 | End: 310 |
| Size | 20 | 20 | 140 |
| Primer 2 details |
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| Sequence | AAGAGGCAGTCTGTTCCACC | GGTTTTAGGCCCCTTTCCGA | Start: 74 |
| Tm | 59.602 | 59.961 | End: 190 |
| Size | 20 | 20 | 117 |
| Primer 3 details |
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| Sequence | CGGAAAGGGGCCTAAAACCA | ATGCTACGGAAGAACGCTGT | Start: 172 |
| Tm | 60.251 | 59.753 | End: 310 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
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| Sequence | TCGGAAAGGGGCCTAAAACC | CCATGCTACGGAAGAACGCT | Start: 171 |
| Tm | 59.961 | 60.46 | End: 312 |
| Size | 20 | 20 | 142 |
| Primer 5 details |
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| Sequence | AGAGGCAGTCTGTTCCACCT | GGTTTTAGGCCCCTTTCCGA | Start: 75 |
| Tm | 60.473 | 59.961 | End: 190 |
| Size | 20 | 20 | 116 |
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