ssr_primer
Primer Details for TRINITY_DN82098_c1_g1_i6 |
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| (A)10tcgtggcatttcaag(A)24tccgaagaaatcactaggagctcaactcgctcagtggtcatagaaaattt(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAGCGTAACAGCACCAAA | GCTCCCACCAGTCGTGTATC | Start: 697 |
| Tm | 59.97 | 60.179 | End: 888 |
| Size | 20 | 20 | 192 |
| Primer 2 details |
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| Sequence | GGCACGTCACGATCGTAGAA | TTTGGTGCTGTTACGCTTGC | Start: 609 |
| Tm | 60.179 | 59.97 | End: 716 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
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| Sequence | GCTGTCATCGATCCCCACAT | GCTCGTGATGACTTTGCACC | Start: 2378 |
| Tm | 59.893 | 59.833 | End: 2524 |
| Size | 20 | 20 | 147 |
| Primer 4 details |
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| Sequence | ATGCTGTCATCGATCCCCAC | GCTCGTGATGACTTTGCACC | Start: 2376 |
| Tm | 59.893 | 59.833 | End: 2524 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
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| Sequence | GCTGTCATCGATCCCCACAT | TCGTGATGACTTTGCACCGT | Start: 2378 |
| Tm | 59.893 | 60.25 | End: 2522 |
| Size | 20 | 20 | 145 |
(T)10gaaggcgagtaagaaggataacgtattttttcttg(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAGCGTAACAGCACCAAA | GCTCCCACCAGTCGTGTATC | Start: 697 |
| Tm | 59.97 | 60.179 | End: 888 |
| Size | 20 | 20 | 192 |
| Primer 2 details |
|---|
| Sequence | GGCACGTCACGATCGTAGAA | TTTGGTGCTGTTACGCTTGC | Start: 609 |
| Tm | 60.179 | 59.97 | End: 716 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
|---|
| Sequence | GCTGTCATCGATCCCCACAT | GCTCGTGATGACTTTGCACC | Start: 2378 |
| Tm | 59.893 | 59.833 | End: 2524 |
| Size | 20 | 20 | 147 |
| Primer 4 details |
|---|
| Sequence | ATGCTGTCATCGATCCCCAC | GCTCGTGATGACTTTGCACC | Start: 2376 |
| Tm | 59.893 | 59.833 | End: 2524 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | GCTGTCATCGATCCCCACAT | TCGTGATGACTTTGCACCGT | Start: 2378 |
| Tm | 59.893 | 60.25 | End: 2522 |
| Size | 20 | 20 | 145 |
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