ssr_primer
Primer Details for TRINITY_DN82078_c1_g1_i5 |
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| (A)28cttgtcacagttgggtgggagggctaaatcttcaccaggcaacagat(C)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCGATCGATCTCACCGAAC | TCCTGGCTGCACATACACTG | Start: 235 |
| Tm | 59.97 | 60.036 | End: 449 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
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| Sequence | AGCGATCGATCTCACCGAAC | GGTTTTGCCCACTTAGCAGC | Start: 235 |
| Tm | 59.97 | 60.038 | End: 343 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
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| Sequence | AGGGCATGAGTACCAACAGC | ACATACACCCAAGCTCTGCC | Start: 1050 |
| Tm | 60.035 | 60.035 | End: 1243 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
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| Sequence | GCCTCCACAGCAGTGTATGT | AGATGCCAGCTGGTGAAGTC | Start: 420 |
| Tm | 60.036 | 60.036 | End: 576 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
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| Sequence | CAGTGTATGTGCAGCCAGGA | AGATGCCAGCTGGTGAAGTC | Start: 430 |
| Tm | 60.036 | 60.036 | End: 576 |
| Size | 20 | 20 | 147 |
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