ssr_primer
Primer Details for TRINITY_DN82066_c2_g1_i10 |
|---|
| (A)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGCGCGCACGATTGATAC | CATGGCAAATAGGGGGCTCA | Start: 1744 |
| Tm | 60.042 | 60.106 | End: 1925 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | CATTGCGCGCACGATTGATA | CATGGCAAATAGGGGGCTCA | Start: 1743 |
| Tm | 60.042 | 60.106 | End: 1925 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | TCCAAAGCAGCGACTTGAGT | CATGGCAAATAGGGGGCTCA | Start: 1685 |
| Tm | 59.893 | 60.106 | End: 1925 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | TTTCATATTTGGCGCCGTGC | CATGGCAAATAGGGGGCTCA | Start: 1724 |
| Tm | 60.179 | 60.106 | End: 1925 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | TGCCATCACGTCCTCACAAT | TCCGGGGTAAGGTTGACTCA | Start: 223 |
| Tm | 59.675 | 60.179 | End: 354 |
| Size | 20 | 20 | 132 |
(A)35: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTGCGCGCACGATTGATAC | CATGGCAAATAGGGGGCTCA | Start: 1744 |
| Tm | 60.042 | 60.106 | End: 1925 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | CATTGCGCGCACGATTGATA | CATGGCAAATAGGGGGCTCA | Start: 1743 |
| Tm | 60.042 | 60.106 | End: 1925 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | TCCAAAGCAGCGACTTGAGT | CATGGCAAATAGGGGGCTCA | Start: 1685 |
| Tm | 59.893 | 60.106 | End: 1925 |
| Size | 20 | 20 | 241 |
| Primer 4 details |
|---|
| Sequence | TTTCATATTTGGCGCCGTGC | CATGGCAAATAGGGGGCTCA | Start: 1724 |
| Tm | 60.179 | 60.106 | End: 1925 |
| Size | 20 | 20 | 202 |
| Primer 5 details |
|---|
| Sequence | TGCCATCACGTCCTCACAAT | TCCGGGGTAAGGTTGACTCA | Start: 223 |
| Tm | 59.675 | 60.179 | End: 354 |
| Size | 20 | 20 | 132 |
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