ssr_primer
Primer Details for TRINITY_DN82032_c2_g1_i7 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGCTGGTGTCGGAAAAAC | CCTTCACGTGTTCTTTCGCC | Start: 3778 |
| Tm | 59.97 | 59.765 | End: 3887 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | CCTTTTTGGTGGTGCTGGTG | CCTTCACGTGTTCTTTCGCC | Start: 3768 |
| Tm | 59.895 | 59.765 | End: 3887 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | ACTTGTGAACAATCAGCGCG | ATGCTGCGGCTCCATTTACT | Start: 3155 |
| Tm | 59.764 | 60.107 | End: 3305 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | ACTTGTGAACAATCAGCGCG | ACTAATGCTGCGGCTCCATT | Start: 3155 |
| Tm | 59.764 | 60.107 | End: 3309 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
|---|
| Sequence | GCGCGAATGATGGCAATGAA | ATGCTGCGGCTCCATTTACT | Start: 3170 |
| Tm | 60.249 | 60.107 | End: 3305 |
| Size | 20 | 20 | 136 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGCTGGTGTCGGAAAAAC | CCTTCACGTGTTCTTTCGCC | Start: 3778 |
| Tm | 59.97 | 59.765 | End: 3887 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | CCTTTTTGGTGGTGCTGGTG | CCTTCACGTGTTCTTTCGCC | Start: 3768 |
| Tm | 59.895 | 59.765 | End: 3887 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | ACTTGTGAACAATCAGCGCG | ATGCTGCGGCTCCATTTACT | Start: 3155 |
| Tm | 59.764 | 60.107 | End: 3305 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | ACTTGTGAACAATCAGCGCG | ACTAATGCTGCGGCTCCATT | Start: 3155 |
| Tm | 59.764 | 60.107 | End: 3309 |
| Size | 20 | 20 | 155 |
| Primer 5 details |
|---|
| Sequence | GCGCGAATGATGGCAATGAA | ATGCTGCGGCTCCATTTACT | Start: 3170 |
| Tm | 60.249 | 60.107 | End: 3305 |
| Size | 20 | 20 | 136 |
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