ssr_primer
Primer Details for TRINITY_DN82017_c3_g4_i2 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGGTCCAAGTTCGTTTTT | GCCGAGTTTCCTCCCTTCAA | Start: 311 |
| Tm | 59.972 | 59.964 | End: 529 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
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| Sequence | AAGCAGAGAGGAACCAAGCC | AAAAACGAACTTGGACCGCG | Start: 106 |
| Tm | 59.963 | 59.972 | End: 330 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
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| Sequence | TTCGTACTCAATGGACGCCC | AAAAACGAACTTGGACCGCG | Start: 223 |
| Tm | 60.109 | 59.972 | End: 330 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | TTTTCCGGCTTTTGGCTTGG | GCCGAGTTTCCTCCCTTCAA | Start: 276 |
| Tm | 59.894 | 59.964 | End: 529 |
| Size | 20 | 20 | 254 |
| Primer 5 details |
|---|
| Sequence | TCCTGTTTAACGCGGTCCAA | GCCGAGTTTCCTCCCTTCAA | Start: 301 |
| Tm | 59.894 | 59.964 | End: 529 |
| Size | 20 | 20 | 229 |
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