ssr_primer
Primer Details for TRINITY_DN82000_c4_g1_i2 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTCCGACCTCTACCTCC | TGTGGGTCGCACATTCGTAA | Start: 2130 |
| Tm | 60.034 | 59.966 | End: 2367 |
| Size | 20 | 20 | 238 |
| Primer 2 details |
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| Sequence | TCCACGTACGCTTCCTTCAC | GGAGGTAGAGGTCGGAGTGT | Start: 1970 |
| Tm | 60.039 | 60.034 | End: 2149 |
| Size | 20 | 20 | 180 |
| Primer 3 details |
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| Sequence | CGGTGAGCAATTCTTCCCCT | GCCAGACAGACGCTTTGTTG | Start: 307 |
| Tm | 60.035 | 60.041 | End: 414 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
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| Sequence | ACCCCTCTCTGCCATCTCAT | CGAACATGCTGCCACTTACG | Start: 1258 |
| Tm | 60.03 | 59.905 | End: 1527 |
| Size | 20 | 20 | 270 |
| Primer 5 details |
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| Sequence | CACGTTCGGATAGCATGGGA | ATGAGATGGCAGAGAGGGGT | Start: 1005 |
| Tm | 59.896 | 60.03 | End: 1277 |
| Size | 20 | 20 | 273 |
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