ssr_primer
Primer Details for TRINITY_DN81996_c5_g3_i4 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTTTCGCAGCGATGGATG | ATGAACATTAATGCCGGCGC | Start: 396 |
| Tm | 59.973 | 59.969 | End: 552 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
|---|
| Sequence | TGCCTGCGAGATGTGAGTTT | AGGCTCTCACAAAGTCTGGC | Start: 1352 |
| Tm | 59.965 | 59.964 | End: 1626 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | TGCCTGCGAGATGTGAGTTT | CACAGCAGGATGGCACTACA | Start: 1352 |
| Tm | 59.965 | 60.036 | End: 1593 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | TGCCTGCGAGATGTGAGTTT | ACATCGAGAAACGCTGAGCA | Start: 1352 |
| Tm | 59.965 | 60.038 | End: 1541 |
| Size | 20 | 20 | 190 |
| Primer 5 details |
|---|
| Sequence | AGCCGTGGGATTGTTGCTTA | ACATCGAGAAACGCTGAGCA | Start: 1290 |
| Tm | 59.962 | 60.038 | End: 1541 |
| Size | 20 | 20 | 252 |
(AT)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGTTTCGCAGCGATGGATG | ATGAACATTAATGCCGGCGC | Start: 396 |
| Tm | 59.973 | 59.969 | End: 552 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
|---|
| Sequence | TGCCTGCGAGATGTGAGTTT | AGGCTCTCACAAAGTCTGGC | Start: 1352 |
| Tm | 59.965 | 59.964 | End: 1626 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | TGCCTGCGAGATGTGAGTTT | CACAGCAGGATGGCACTACA | Start: 1352 |
| Tm | 59.965 | 60.036 | End: 1593 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | TGCCTGCGAGATGTGAGTTT | ACATCGAGAAACGCTGAGCA | Start: 1352 |
| Tm | 59.965 | 60.038 | End: 1541 |
| Size | 20 | 20 | 190 |
| Primer 5 details |
|---|
| Sequence | AGCCGTGGGATTGTTGCTTA | ACATCGAGAAACGCTGAGCA | Start: 1290 |
| Tm | 59.962 | 60.038 | End: 1541 |
| Size | 20 | 20 | 252 |
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