ssr_primer
Primer Details for TRINITY_DN81960_c1_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCCTTTCATCCCTGCCAT | GCTTTCGCAACCAACACCTT | Start: 3 |
| Tm | 60.106 | 59.898 | End: 117 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | TGTTCGTAGCTGAAACGCCT | AGCAAGAGAACCAAGGCGTT | Start: 489 |
| Tm | 59.967 | 60.179 | End: 638 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
|---|
| Sequence | AACGCCTTGGTTCTCTTGCT | TTGAAGGTGAGGCCAAGGAC | Start: 619 |
| Tm | 60.179 | 59.89 | End: 853 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | AACGCCTTGGTTCTCTTGCT | TATGTGAGTGCGCCGATTGA | Start: 619 |
| Tm | 60.179 | 59.825 | End: 816 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | GAACGAGGCTAACTGGGGTT | AGGCGTTTCAGCTACGAACA | Start: 233 |
| Tm | 59.676 | 59.967 | End: 508 |
| Size | 20 | 20 | 276 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTCCTTTCATCCCTGCCAT | GCTTTCGCAACCAACACCTT | Start: 3 |
| Tm | 60.106 | 59.898 | End: 117 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | TGTTCGTAGCTGAAACGCCT | AGCAAGAGAACCAAGGCGTT | Start: 489 |
| Tm | 59.967 | 60.179 | End: 638 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
|---|
| Sequence | AACGCCTTGGTTCTCTTGCT | TTGAAGGTGAGGCCAAGGAC | Start: 619 |
| Tm | 60.179 | 59.89 | End: 853 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | AACGCCTTGGTTCTCTTGCT | TATGTGAGTGCGCCGATTGA | Start: 619 |
| Tm | 60.179 | 59.825 | End: 816 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | GAACGAGGCTAACTGGGGTT | AGGCGTTTCAGCTACGAACA | Start: 233 |
| Tm | 59.676 | 59.967 | End: 508 |
| Size | 20 | 20 | 276 |
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