ssr_primer
Primer Details for TRINITY_DN81948_c4_g1_i1 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATGGACAGCACCTACCCT | TCCCCATGGAGGCAATAGGA | Start: 1834 |
| Tm | 60.253 | 60.029 | End: 2016 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
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| Sequence | TCATGGACAGCACCTACCCT | TTGCTACCGAAGTGCTCGTT | Start: 1834 |
| Tm | 60.253 | 59.967 | End: 2060 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
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| Sequence | TCCTATTGCCTCCATGGGGA | TGTCCAGGAGCATCAAGTGG | Start: 1997 |
| Tm | 60.029 | 59.674 | End: 2137 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
|---|
| Sequence | TCATGGACAGCACCTACCCT | AGGCAATAGGAGTTCCACGC | Start: 1834 |
| Tm | 60.253 | 60.108 | End: 2007 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
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| Sequence | GCGTGGAACTCCTATTGCCT | TGTCCAGGAGCATCAAGTGG | Start: 1988 |
| Tm | 60.108 | 59.674 | End: 2137 |
| Size | 20 | 20 | 150 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATGGACAGCACCTACCCT | TCCCCATGGAGGCAATAGGA | Start: 1834 |
| Tm | 60.253 | 60.029 | End: 2016 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | TCATGGACAGCACCTACCCT | TTGCTACCGAAGTGCTCGTT | Start: 1834 |
| Tm | 60.253 | 59.967 | End: 2060 |
| Size | 20 | 20 | 227 |
| Primer 3 details |
|---|
| Sequence | TCCTATTGCCTCCATGGGGA | TGTCCAGGAGCATCAAGTGG | Start: 1997 |
| Tm | 60.029 | 59.674 | End: 2137 |
| Size | 20 | 20 | 141 |
| Primer 4 details |
|---|
| Sequence | TCATGGACAGCACCTACCCT | AGGCAATAGGAGTTCCACGC | Start: 1834 |
| Tm | 60.253 | 60.108 | End: 2007 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | GCGTGGAACTCCTATTGCCT | TGTCCAGGAGCATCAAGTGG | Start: 1988 |
| Tm | 60.108 | 59.674 | End: 2137 |
| Size | 20 | 20 | 150 |
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