ssr_primer
Primer Details for TRINITY_DN81916_c1_g1_i5 |
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| (A)14g(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGCATGAACTTCGCCAGT | GGCTCGGCATCTCGTGATAA | Start: 1398 |
| Tm | 59.965 | 59.968 | End: 1650 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
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| Sequence | TTATCACGAGATGCCGAGCC | AGGGTTCGCAAATGACGGAT | Start: 1631 |
| Tm | 59.968 | 60.035 | End: 1891 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
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| Sequence | TTTGGCTAGCCTCAGTGAGC | GTCGACACACCCTGTTCGAT | Start: 2724 |
| Tm | 60.036 | 60.039 | End: 2939 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | CCGAGTCGAGCTCAAATCGA | TTATGCTAGGGCTGTGCGAG | Start: 212 |
| Tm | 59.901 | 59.895 | End: 469 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
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| Sequence | CCGAGTCGAGCTCAAATCGA | TTGGGGCTTTATGCTAGGGC | Start: 212 |
| Tm | 59.901 | 60.106 | End: 477 |
| Size | 20 | 20 | 266 |
(GAT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGCATGAACTTCGCCAGT | GGCTCGGCATCTCGTGATAA | Start: 1398 |
| Tm | 59.965 | 59.968 | End: 1650 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TTATCACGAGATGCCGAGCC | AGGGTTCGCAAATGACGGAT | Start: 1631 |
| Tm | 59.968 | 60.035 | End: 1891 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TTTGGCTAGCCTCAGTGAGC | GTCGACACACCCTGTTCGAT | Start: 2724 |
| Tm | 60.036 | 60.039 | End: 2939 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | CCGAGTCGAGCTCAAATCGA | TTATGCTAGGGCTGTGCGAG | Start: 212 |
| Tm | 59.901 | 59.895 | End: 469 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
|---|
| Sequence | CCGAGTCGAGCTCAAATCGA | TTGGGGCTTTATGCTAGGGC | Start: 212 |
| Tm | 59.901 | 60.106 | End: 477 |
| Size | 20 | 20 | 266 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGCATGAACTTCGCCAGT | GGCTCGGCATCTCGTGATAA | Start: 1398 |
| Tm | 59.965 | 59.968 | End: 1650 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TTATCACGAGATGCCGAGCC | AGGGTTCGCAAATGACGGAT | Start: 1631 |
| Tm | 59.968 | 60.035 | End: 1891 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | TTTGGCTAGCCTCAGTGAGC | GTCGACACACCCTGTTCGAT | Start: 2724 |
| Tm | 60.036 | 60.039 | End: 2939 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | CCGAGTCGAGCTCAAATCGA | TTATGCTAGGGCTGTGCGAG | Start: 212 |
| Tm | 59.901 | 59.895 | End: 469 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
|---|
| Sequence | CCGAGTCGAGCTCAAATCGA | TTGGGGCTTTATGCTAGGGC | Start: 212 |
| Tm | 59.901 | 60.106 | End: 477 |
| Size | 20 | 20 | 266 |
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