ssr_primer
Primer Details for TRINITY_DN81894_c5_g3_i1 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTCGCACATGCCAAAAGC | GGGATATCGAGCTGACACGG | Start: 1646 |
| Tm | 60.039 | 60.039 | End: 1746 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TTCTCGCACATGCCAAAAGC | GGGGATATCGAGCTGACACG | Start: 1646 |
| Tm | 60.039 | 60.039 | End: 1747 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | ACAGCTCAACGCACCCATAA | TTGCAAACCTCCCTTTCCGT | Start: 1363 |
| Tm | 59.964 | 60.106 | End: 1534 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | ACAGCTCAACGCACCCATAA | GCTGTTCTTGCTATCCCGGT | Start: 1363 |
| Tm | 59.964 | 60.108 | End: 1464 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | ACAGCTCAACGCACCCATAA | CGTGTGGCCCGATCTTAACT | Start: 1363 |
| Tm | 59.964 | 60.108 | End: 1517 |
| Size | 20 | 20 | 155 |
(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTCGCACATGCCAAAAGC | GGGATATCGAGCTGACACGG | Start: 1646 |
| Tm | 60.039 | 60.039 | End: 1746 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TTCTCGCACATGCCAAAAGC | GGGGATATCGAGCTGACACG | Start: 1646 |
| Tm | 60.039 | 60.039 | End: 1747 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | ACAGCTCAACGCACCCATAA | TTGCAAACCTCCCTTTCCGT | Start: 1363 |
| Tm | 59.964 | 60.106 | End: 1534 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | ACAGCTCAACGCACCCATAA | GCTGTTCTTGCTATCCCGGT | Start: 1363 |
| Tm | 59.964 | 60.108 | End: 1464 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | ACAGCTCAACGCACCCATAA | CGTGTGGCCCGATCTTAACT | Start: 1363 |
| Tm | 59.964 | 60.108 | End: 1517 |
| Size | 20 | 20 | 155 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCTCGCACATGCCAAAAGC | GGGATATCGAGCTGACACGG | Start: 1646 |
| Tm | 60.039 | 60.039 | End: 1746 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TTCTCGCACATGCCAAAAGC | GGGGATATCGAGCTGACACG | Start: 1646 |
| Tm | 60.039 | 60.039 | End: 1747 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | ACAGCTCAACGCACCCATAA | TTGCAAACCTCCCTTTCCGT | Start: 1363 |
| Tm | 59.964 | 60.106 | End: 1534 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | ACAGCTCAACGCACCCATAA | GCTGTTCTTGCTATCCCGGT | Start: 1363 |
| Tm | 59.964 | 60.108 | End: 1464 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | ACAGCTCAACGCACCCATAA | CGTGTGGCCCGATCTTAACT | Start: 1363 |
| Tm | 59.964 | 60.108 | End: 1517 |
| Size | 20 | 20 | 155 |
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