Primer Details for TRINITY_DN81829_c2_g4_i1 |
|---|
| (G)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACCCTAGAGAGTACGATTTTCA | GCCTGCCTGCCTCTTTCTTT | Start: 135 |
| Tm | 58.741 | 60.897 | End: 412 |
| Size | 24 | 20 | 278 |
| Primer 2 details |
|---|
| Sequence | CGGGGAAAATATGTTTAAATTGCGT | TGCTTATCTGCCGTGCTGAG | Start: 6 |
| Tm | 59.192 | 60.461 | End: 112 |
| Size | 25 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | CGGGGAAAATATGTTTAAATTGCGT | CTGCTTATCTGCCGTGCTGA | Start: 6 |
| Tm | 59.192 | 60.461 | End: 113 |
| Size | 25 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | CGGGGAAAATATGTTTAAATTGCGT | TATCTGCCGTGCTGAGGAAG | Start: 6 |
| Tm | 59.192 | 59.538 | End: 108 |
| Size | 25 | 20 | 103 |
| Primer 5 details |
|---|
| Sequence | CGGGGAAAATATGTTTAAATTGCGT | CTTATCTGCCGTGCTGAGGA | Start: 6 |
| Tm | 59.192 | 59.538 | End: 110 |
| Size | 25 | 20 | 105 |
