ssr_primer
Primer Details for TRINITY_DN81796_c2_g6_i5 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGAACGGAGGGCTTGTTAA | GCGGTGTCGTGGATTTATGC | Start: 1191 |
| Tm | 59.963 | 59.972 | End: 1340 |
| Size | 20 | 20 | 150 |
| Primer 2 details |
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| Sequence | TTCGACTACTCCGGGTGCTA | ACTGCCGCCATATAGAAGCC | Start: 8 |
| Tm | 60.035 | 59.965 | End: 115 |
| Size | 20 | 20 | 108 |
| Primer 3 details |
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| Sequence | GGCTTCTATATGGCGGCAGT | AGGCGGTTCTGCGTGAAATA | Start: 96 |
| Tm | 59.965 | 60.037 | End: 302 |
| Size | 20 | 20 | 207 |
| Primer 4 details |
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| Sequence | AGAACTTTCGGCCTCTGAGC | AGGCGGTTCTGCGTGAAATA | Start: 33 |
| Tm | 60.037 | 60.037 | End: 302 |
| Size | 20 | 20 | 270 |
| Primer 5 details |
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| Sequence | GGGAACGGAGGGCTTGTTAA | GTTTTTCCTCAGCACGGCAG | Start: 1191 |
| Tm | 59.963 | 60.04 | End: 1413 |
| Size | 20 | 20 | 223 |
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